« 於: 三月 01, 2017, 12:05:32 am »
To get the unmapped reads from a bam file use :
samtools view -f 4 file.bam > unmapped.sam, the output will be in sam
to get the output in bam use : samtools view -b -f 4 file.bam > unmapped.bam
To get only the mapped reads use the parameter 'F', which works like -v of grep and skips the alignments for a specific flag.
samtools view -b -F 4 file.bam > mapped.bam
